Ukuqapha okuku-inthanethi kokuqukethwe kwe-glucose ukuze kudliwe ngesikhathi sangempela ukuze kuqinisekiswe ukuqedwa kahle kwenqubo yokuvutshelwa.
Ubunjiniyela be-Biofermentation bungenye yezingxenye ezibalulekile zobunjiniyela besimanje be-biopharmaceutical, ukuthola imikhiqizo efiselekayo ye-biochemical ngenqubo yokukhula kwama-microorganisms.Inqubo yokukhula kwama-microbial ihlanganisa izigaba ezine: isigaba sokuzivumelanisa nezimo, isigaba sokungena, isigaba sokuma, nesigaba sokufa.Ngesikhathi sokumiswa, inani elikhulu lemikhiqizo ye-metabolic liyanqwabelana.Lesi futhi isikhathi lapho imikhiqizo ivunwa ngokusabela okuningi.Uma lesi sigaba sesidlulile futhi isigaba sokufa sesingenile, kokubili ukusebenza kwamangqamuzana amagciwane kanye nokuhlanzeka kwemikhiqizo kuzothinteka kakhulu.Ngenxa yobunkimbinkimbi bokusabela kwebhayoloji, ukuphinda kwenqubo yokuvutshelwa kubi, futhi nokulawulwa kwekhwalithi kuyinselele.Njengoba inqubo ikhuphuka isuka elabhorethri iye esikalini sokuhlola, futhi isuka esilinganisweni sokulinga iye emkhiqizweni omkhulu, okungajwayelekile ekuphenduleni kungenzeka kalula.Ukuqinisekisa ukuthi ukuvutshelwa kugcinwa esigabeni sokunganyakazi isikhathi eside kuyindaba ekhathaza kakhulu lapho kukhuliswa ubunjiniyela bokuvutshelwa.
Ukuqinisekisa ukuthi uhlobo lwe-microbial luhlala lusesigabeni sokukhula esinamandla futhi esizinzile ngesikhathi sokuvutshelwa, kubalulekile ukugcina okuqukethwe kwama-metabolites wamandla adingekayo njengeglucose.Ukusebenzisa i-spectroscopy eku-inthanethi ukuqapha okuqukethwe kwe-glucose kumhluzi wokuvutshelwa ngesikhathi sangempela kuyindlela efanelekile yobuchwepheshe yokulawula inqubo ye-biofermentation: ukuthatha izinguquko ekugxilweni kwe-glucose njengendlela yokwengeza nokunquma isimo sobunzima be-microbial.Uma okuqukethwe kuwela ngaphansi komkhawulo omisiwe, ukwenezela kungenziwa ngokushesha ngokusekelwe emiphumeleni yokuqapha, okuthuthukisa kakhulu ikhwalithi nokusebenza kahle kwe-biofermentation.Njengoba kukhonjisiwe kumdwebo ongezansi, igatsha eliseceleni lithathwa ethangini elincane lokuvutshelwa.I-spectroscopy probe ithola amasiginali oketshezi lokuvutshelwa ngesikhathi sangempela ngokusebenzisa i-circulation pool, ekugcineni ivumele ukutholakala kokugxiliswa kweglucose oketshezini oluvutshelwayo kuze kube ngaphansi ku-3 ‰.
Ngakolunye uhlangothi, uma isampula elingaxhunyiwe ku-inthanethi lomhluzi wokuvubela nokuhlolwa kwaselabhorethri kusetshenziswa ukulawula inqubo, imiphumela ebambezelekile yokuthola ingase iphuthe isikhathi esifanelekile sokwengeza.Ngaphezu kwalokho, inqubo yesampula ingase ithinte uhlelo lokuvutshelwa, njengokungcoliswa amagciwane angaphandle.
Isikhathi sokuthumela: Dec-07-2023