Ukuqapha ku-inthanethi kuqinisekisa ukuthi okuqukethwe kwe-substrate kungaphansi komkhawulo, kuqinisekisa umsebenzi we-enzyme yebhayoloji kuyo yonke inqubo, futhi kukhulisa izinga lokusabela kwe-hydrolysis.
Amakhompiyutha e-Amide abalulekile ekuhlanganiseni kwe-organic synthesis namakhemikhali futhi asetshenziswa kakhulu kwezokwelapha, izibulala-zinambuzane, ukudla, ukuvikelwa kwemvelo, ukukhiqizwa kukawoyela neminye imikhakha.Ukusabela kwe-hydrolysis kweqembu le-nitrile eqenjini le-amide kungenye yezindlela ezibalulekile zokulungiselela ama-amide compounds embonini.
I-biocatalyst isetshenziselwa inqubo yokuhlanganiswa okuluhlaza kwenhlanganisela ethile ye-amide, futhi umsebenzi wayo uthinteka kakhulu ekugxilweni kwe-substrate nomkhiqizo ohlelweni.Uma ukuhlushwa kwe-substrate kuphezulu kakhulu, i-catalyst izovalwa kalula, okwenza kube nzima ukuqhubeka nokusabela kokuhlanganiswa;uma ukugxila komkhiqizo kuphezulu kakhulu, kuzophinde kuholele ekuqoqweni kwe-substrate kanye nokusebenza kahle kokuhlanganiswa okuphansi.Ukuze kuqinisekiswe umsebenzi ofanele wama-enzyme e-biological catalysts ekuphenduleni kokuhlanganiswa, izindlela zobuchwepheshe ezisebenzayo ziyadingeka ukuze kuqashwe futhi impendulo ilungise ukugxila kwe-nitrile substrates kanye nemikhiqizo ye-amide ngesikhathi sangempela phakathi nenqubo yokusabela.
Njengamanje, izindlela ezifana nokusampula ngezikhathi ezimisiwe kanye nokwenza i-gas chromatography-mass spectrometry ngemva kwesampula yokwelashwa kwangaphambili zivame ukusetshenziswa ukuze kutholwe i-substrate nokuqukethwe komkhiqizo ohlelweni lokusabela.Imiphumela yokutholwa ungaxhunyiwe ku-inthanethi ibambezeleka, isimo samanje sokusabela asikwazi ukwaziwa ngesikhathi sangempela, futhi kunzima ukulawula impendulo nokulungiswa kokuqukethwe kwe-substrate, futhi ithuba elingcono kakhulu lokuphakela lingase liphuthelwe.Ubuchwepheshe bokuhlaziya i-spectral eku-inthanethi bunobuhle besivinini sokutholwa ngokushesha futhi asikho isidingo sokwelashwa kwangaphambili kwesampula.Ingabona ukuhlaziya okusheshayo, kwesikhathi sangempela, in-situ nokukhaliphile kwesistimu yokusabela, futhi kunezinzuzo eziyingqayizivele ekuhlanganiseni okuluhlaza kwezinhlanganisela ze-amide.
Isithombe esingenhla sibonisa ukuqapha ku-inthanethi kwenqubo yokulungisa i-acrylamide ngokusabela kwe-bioenzymatic kwenhlanganisela ethile ye-nitrile.Ukusuka ku-0 kuye ku-t1 ngemva kokuqala kokusabela, izinga lokudla kwe-nitrile eluhlaza likhulu ngokuqhathaniswa, futhi izinga lokunqwabelana lakho kokubili i-substrate nomkhiqizo liyashesha uma kuqhathaniswa.Ku-t1, okuqukethwe kwe-substrate kuseduze nomkhawulo ongaphezulu womkhawulo.Ngalesi sikhathi, izisebenzi ezikhiqizayo zehlisa izinga lokuphakelwa kwezinto zokusetshenziswa ukuze kugcinwe ukugxiliswa kwe-substrate ohlelweni lokusabela ngaphakathi kwebanga elilawulekayo, futhi umkhiqizo usengaqongelela ngokushesha.Ekugcineni, lapho ukusabela kuqhubeka kusikhathi esingu-t2, okuqukethwe komkhiqizo kunqwabelana kuye ezingeni eliqondiwe, futhi abasebenzi abakhiqizayo bayayeka ukungeza izinto zokusetshenziswa ze-nitrile.Ngemuva kwalokho, izinga le-substrate lisondela ku-zero futhi okuqukethwe komkhiqizo nakho kuthanda ukuzinza.Phakathi nayo yonke inqubo yokukhiqiza eqhubekayo, ukuqapha ku-inthanethi kuqinisekisa ukuthi ukusabela kwe-enzyme yebhayoloji kuqhubeka kahle.
Ekuhlanganiseni okukhulu, ubuchwepheshe bokuqapha ku-inthanethi bubaluleke kakhulu.Ulwazi lwesikhathi sangempela lwe-substrate nokugxilwa komkhiqizo lungasiza impendulo ukulungisa okuqukethwe kwe-substrate ngaphakathi kwebanga elinengqondo.Ngesikhathi senqubo yokusabela, ingakhulisa umsebenzi we-enzyme ye-biological catalyst, ithuthukise ukusebenza kahle kokusabela kokuhlanganiswa, futhi isize ukulawula imingcele yenqubo esimweni esifanele.Nweba impilo yesevisi yama-enzyme e-biological catalysts futhi wandise izinzuzo.
Isikhathi sokuthumela: Jan-23-2024